LOCUS       pLV-[hsa-miR-106a_18b_20b_19b-2_92a-2]  9761 bp    DNA     circular     12-APR-2007
SOURCE      
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7682..8542
                     /label=Amp(R)
     promoter        8743..9283
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             6287..6521
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(7096..7551)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    6593..6723
                     /label=SV40 pA
     misc_feature    6724..6937
                     /label=SV40 ori
     misc_feature    5608..6204
                     /label=WPRE
     promoter        1933..2328
                     /label=human EF1a promoter
     intron          2361..4226
                     /label=intron
     CDS             4273..5598
                     /label=rPuro (RFP Puro)
     misc_RNA        3110..4016
                     /label=hsa-mir-106a_18b_20b_19b-2_92a-2 precursor
BASE COUNT     2329 a      2457 c      2617 g      2358 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgaggatcct gttttaacca 
     3121 ggtgagtctg catggatctg tgaggacgga aaagaagagc tcctggaaga cttaaaattt 
     3181 tgctacagga ataggccttg gccatgtaaa agtgcttaca gtgcaggtag ctttttgaga 
     3241 tctactgcaa tgtaagcact tcttacatta ccatggtgat ttagtcaatg gctactgaga 
     3301 actgtagttt gtgcataatt aagtagttga tgcttttgag ctgcttctta taatgtgtct 
     3361 cttgtgttaa ggtgcatcta gtgcagttag tgaagcagct tagaatctac tgccctaaat 
     3421 gccccttctg gcacaggctg cctaatatac agcattttaa aagtatgcct tgagtagtaa 
     3481 tttgaatagg acacatttca gtggtttgtt ttttgccttt ttattgtttg ttgggaacag 
     3541 atggtgggga ctgtgcagtg tacagttgtg tacagaggat aagattgggt cctagtagta 
     3601 ccaaagtgct catagtgcag gtagttttgg catgactcta ctgtagtatg ggcacttcca 
     3661 gtactcttgg ataacaaatc tcttgttgat ggagagaata ttcaaagaca ttgctactta 
     3721 caattagttt tgcaggtttg catttcagcg tatatatgta tatgtggctg tgcaaatcca 
     3781 tgcaaaactg attgtgataa tgtgtgcttc ctacgtctgt gtgaacacac cttcatgcgt 
     3841 atctccagca ctcatgccca ttcatccctg ggtggggatt tgttgcatta cttgtgttct 
     3901 atataaagta ttgcacttgt cccggcctgt ggaagaaagg aggattttta tcgtcttctt 
     3961 attttaactt ttaaaagccg taagttctga tatttagtca ttgtaaaatg atctgtgcta 
     4021 gctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt 
     4081 ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct 
     4141 ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg 
     4201 ttcaaagttt ttttcttcca tttcaggtgt cgtgaaaact acccctctag agtcgagcta 
     4261 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc 
     4321 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc 
     4381 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt 
     4441 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc 
     4501 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc 
     4561 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc 
     4621 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc 
     4681 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac 
     4741 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc 
     4801 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc 
     4861 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg 
     4921 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag 
     4981 gacccaccgg tcgccaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac 
     5041 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc 
     5101 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc 
     5161 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg 
     5221 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc 
     5281 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg 
     5341 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac 
     5401 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc 
     5461 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc 
     5521 ggcttcaccg tcaccgccga cgtcgagtgc ccgaaggacc gcgcgacctg gtgcatgacc 
     5581 cgcaagcccg gtgcctgagc ggccgcgtcg acaatcaacc tctggattac aaaatttgtg 
     5641 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt 
     5701 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata 
     5761 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg 
     5821 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc 
     5881 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct 
     5941 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt 
     6001 cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg 
     6061 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc 
     6121 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct 
     6181 ccctttgggc cgcctccccg cctggaattc gagctcggta cctttaagac caatgactta 
     6241 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat 
     6301 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca 
     6361 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag 
     6421 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag 
     6481 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct 
     6541 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt 
     6601 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc 
     6661 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt 
     6721 ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc 
     6781 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc 
     6841 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac 
     6901 ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc 
     6961 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg 
     7021 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc 
     7081 tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta 
     7141 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc 
     7201 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt 
     7261 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg 
     7321 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca 
     7381 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct 
     7441 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga 
     7501 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac 
     7561 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat 
     7621 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag 
     7681 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc 
     7741 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc 
     7801 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc 
     7861 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc 
     7921 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt 
     7981 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt 
     8041 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat 
     8101 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct 
     8161 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat 
     8221 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc 
     8281 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg 
     8341 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc 
     8401 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta 
     8461 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc 
     8521 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga 
     8581 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat 
     8641 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat 
     8701 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa 
     8761 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa 
     8821 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt 
     8881 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt 
     8941 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata 
     9001 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt 
     9061 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac 
     9121 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga 
     9181 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg 
     9241 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa 
     9301 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat 
     9361 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc 
     9421 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga 
     9481 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg 
     9541 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta 
     9601 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg 
     9661 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg 
     9721 cgcaattaac cctcactaaa gggaacaaaa gctggagctg c 
//
